E.coli "strain 4" I241E
Description
Technical info
Tilt Series Date: 2012-03-07
Data Taken By: Ariane Briegel
Species / Specimen: Escherichia coli
Strain: RP 437
Tilt Series Settings: Single Axis, tilt range: (-63.0°, 63.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Ų, defocus: -10.0 μm, magnification: 34000x.
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Strain generated by John S. Parkinson
Purification / Growth Conditions / Treatment: cells grown in TB (gentamycin) overnight at 30 degrees. Diluted in fresh buffer and grown to log phase. Then incubated with penicillin for one hour.
Sample Preparation: Vitrobot settings: -4 offset, 3.5 s blot time, no drain, 100 percent humidity
Attached Files
strain4marchseven0017.mrc, Tilt Series (Pixel Size 0.644 nm), 1.1 GB
strain4marchseven0017_part128_2.rec, Reconstruction (Pixel Size 1.288 nm), 516.1 MB
Files
keyimg_aba2012-03-07-12.jpg
Additional details
Identifiers
- DOI
- 10.22002/vk02n-v3y13
- TILT_SERIES_ID
- aba2012-03-07-12
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2012-03-07
- Created
-
2012-03-07
- Updated
-
2012-03-14