Published 2014 | Version v1
Dataset Open

HeLa

  • 1. ROR icon California Institute of Technology

Description

Synchronized, looking for midbodies

Technical info

Tilt Series Date: 2014-12-29

Data Taken By: Rasika Ramdasi

Species / Specimen: HeLa cells

Strain: ATCC

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -10.0 μm, magnification: 18000x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: UCSF tomo

Collaborators and Roles: With Doug Mckay and Katherine Ullman

Sample Preparation: HeLa cells+ siRNA NUP 153+ Thymidine 2mM. 14 hour after release

Attached Files

midbody10005.mrc, Tilt Series (Pixel Size 0.6 nm), 3.4 GB

midbody10005_part121_41.rec, Reconstruction (Pixel Size 2.4 nm), 623.6 MB

Files

keyimg_rra2014-12-29-23.jpg

Files (4.1 GB)

Name Size
md5:0a7380cdab202f78d3c513c1e49b6cb2
1.1 MB Preview Download
md5:fbbf05fd4c854e32916b1d6d54287517
15.7 MB Preview Download
3.4 GB (external) Download
623.6 MB (external) Download

Additional details

Identifiers

DOI
10.22002/vq4hw-p3164
TILT_SERIES_ID
rra2014-12-29-23

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2014-12-29
Created
2015-01-05
Updated
2015-01-08