Saccharomyces cerevisiae - 6210
Contributors
Researcher:
Description
Technical info
Tilt Series Date: 2017-10-25
Data Taken By: Shrawan kumar Mageswaran
Species / Specimen: Unspecified
Strain: None
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 140.0 eV/Ų, defocus: -8.0 μm, magnification: 27500x.
Microscope: Caltech Polara
Acquisition Software: SerialEM
Upload Method: reupload_downloaded
Processing Software Used: IMOD
Collaborators and Roles: Strain from Markus Babst @ University of Utah
Purification / Growth Conditions / Treatment: Grew cells to ~0.6 OD from overnight cultures in YPD.
Sample Preparation: added ~3.5 µL of ~0.2 OD cells for each grid and blotted from behind for ~3 sec. FIB milled on Sep25_2017.
Attached Files
TS3_comb.mrc, Tilt Series (Pixel Size 0.39 nm), 65.9 MB
TS3_4bin_comb_DB.rec, Reconstruction (Pixel Size 0.0 nm), 256.6 MB
Files
keyimg_smk2017-10-25-4.jpg
Additional details
Identifiers
- DOI
- 10.22002/vt1b8-8f090
- TILT_SERIES_ID
- smk2017-10-25-4
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2017-10-25
- Created
-
2018-01-04
- Updated
-
2018-01-05