HeLa CHMP4A+VPS4DN
Contributors
Researchers:
Description
Technical info
Tilt Series Date: 2011-07-21
Data Taken By: Cora Woodward
Species / Specimen: HeLa cells
Strain: ATCC
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -7.0 μm, magnification: 22500x.
Acquisition Software: UCSF tomo
Upload Method: rundir
Processing Software Used: imod
Collaborators and Roles: Constructs provided by Wes Sundquist. Cells transfected, frozen and imaged at Caltech by Cora Woodward
Purification / Growth Conditions / Treatment: whole cells grown on grids and transfected with lipofectamine
Sample Preparation: manual blotting
Attached Files
11jul21_0004_ns.st, Tilt Series (Pixel Size 0.5 nm), 817.6 MB
11jul21_0004_ns_full.rec, Reconstruction (Pixel Size 2.0 nm), 701.9 MB
11jul21_0004_ns_full.tiff, file associated to 3D Image #68318, 898.4 kB
Files
11jul21_0004_ns_full.tiff
- This file is an external reference and not stored directly in this repository. To access its content, please download it and open it locally.
Additional details
Identifiers
- DOI
- 10.22002/vtfeg-yh385
- TILT_SERIES_ID
- cwl2011-07-21-2
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2011-07-21
- Created
-
2015-09-16
- Updated
-
2015-09-16