Caltech Tomography Archive
Published 2015 | Version v1
Dataset Open

immunogoldMtrc_wt

Creators

  • 1. ROR icon California Institute of Technology

Contributors

Researcher:

Description

This is wt made in perfusion chamber and fixed with 2.5% glutaraldehyde and immune-gold labeled for Mtr complex. The grid # is PS123-#3

Technical info

Tilt Series Date: 2015-12-29

Data Taken By: Poorna Subramanian

Species / Specimen: Shewanella oneidensis

Strain: MR-1

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 143.0 eV/Ų, defocus: -6.0 μm, magnification: 34000x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Sahand and Moh, USC

Sample Preparation: The sample was pulled out of Dh2O, was loaded on vitrobot with C side on left. Before blotting added 4ul of water. Blotting conditions: blot force -6, blot time -3.

Attached Files

atlas2_sq150004_full.rec, Reconstruction (Pixel Size 1.28 nm), 445.4 MB

atlas2_sq150004.mrc, Tilt Series (Pixel Size 0.32 nm), 3.4 GB

Files

keyimg_psa2015-12-29-4.jpg

Files (3.9 GB)

Name Size
3.4 GB (external) Download
445.4 MB (external) Download
md5:561c6641e9a59e712bc65d1abb8439a6
1.0 MB Preview Download
md5:0fb352a72f0aaf08f784f7bba2871478
12.2 MB Preview Download

Additional details

Identifiers

DOI
10.22002/vtjny-2h719
TILT_SERIES_ID
psa2015-12-29-4

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2015-12-29
Created
2015-12-30
Updated
2016-01-04