Published 2017 | Version v1
Dataset Open

E.coli minicell/Pul T2SS

Creators

  • 1. ROR icon California Institute of Technology

Contributors

Description

grid box YWC252strain PAP5359 (minCDE knockout)Cells were grown on plate before freezing

Technical info

Tilt Series Date: 2017-05-15

Data Taken By: Yi-Wei Chang

Species / Specimen: Escherichia coli

Strain:

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 130.0 eV/Ų, defocus: -6.0 μm, magnification: 27500x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Strain provided by Ingrid Guilvout in Olivera Francetic lab

ycw2017-05-15-54_slicer18082.jpg: outer membrane double leaflet & peptidoglycan layer ycw2017-05-15-54_slicer18083.jpg: outer membrane double leaflet & peptidoglycan layer

Attached Files

20170515_YWC252b_10059.mrc, Tilt Series (Pixel Size 0.39 nm), 3.4 GB

20170515_YWC252b_10059_full.rec, Reconstruction (Pixel Size 1.56 nm), 534.5 MB

Files

keyimg_ycw2017-05-15-54.jpg

Files (4.0 GB)

Name Size
3.4 GB (external) Download
534.5 MB (external) Download
md5:55d9a54095d46763b56123058d6830f1
1.1 MB Preview Download
md5:3169738fde40cd224b5b4859217fad87
18.3 MB Preview Download
md5:524f3deab4f5b8043d39a6ddbfdfb2b5
264.9 kB Preview Download
md5:5dcd08d67cd6d062c9158fcde867849e
265.3 kB Preview Download

Additional details

Identifiers

DOI
10.22002/vwgma-8t133
TILT_SERIES_ID
ycw2017-05-15-54

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2017-05-15
Created
2017-05-16
Updated
2017-05-30