Caltech Tomography Archive
Published 2015 | Version v1
Dataset Open

wt_perfusion_fixed_immunofluoro

Creators

  • 1. ROR icon California Institute of Technology

Description

This sample is wt cells grown in perfusion set-up. It was imaged on LM for wires, fixed with 4 % formaldehyde for 1 hour, immunofluorescence was done for cytochromes, reimaged, fixed with 2.5% glutaraldehyde for 15 minutes, triple washed with distilled water and saved in distilled water until freezing. The grid was prepared by Sahand on 10/22/15. The grid was frozen on 10/23/15 at Caltech using vitrobot MK4.

Technical info

Tilt Series Date: 2015-10-27

Data Taken By: Poorna Subramanian

Species / Specimen: Shewanella oneidensis

Strain: MR-1

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 130.0 eV/Ų, defocus: -5.0 μm, magnification: 34000x.

Microscope: Caltech Polara

Acquisition Software: Undefined

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Moh and Sahand USC

Sample Preparation: The sample was frozen using vitrobot. C side left. 1.8 ul gold 10nm size was added on opposite side while on vitrobot. conditions: blot force=6, blot time=2.5s, drain time =1s, wait time=0. humidity set 80%. temp 22C. Use 2.5ul gold next time

Attached Files

manual4_sq10-1_part121_8.rec, Reconstruction (Pixel Size 1.28 nm), 445.4 MB

manual4_sq10-1.mrc, Tilt Series (Pixel Size 0.32 nm), 3.4 GB

Files

keyimg_psa2015-10-27-9.jpg

Files (3.9 GB)

Name Size
md5:06d5c7909ba22a325944516cb87c77c7
1.1 MB Preview Download
md5:a85a50085fcc60329db92088e8b1e1e3
14.9 MB Preview Download
3.4 GB (external) Download
445.4 MB (external) Download

Additional details

Identifiers

DOI
10.22002/w4mcb-teq58
TILT_SERIES_ID
psa2015-10-27-9

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2015-10-27
Created
2015-10-27
Updated
2016-01-04