Published 2018 | Version v1
Dataset Open

gh9-3_sq3_cell5

  • 1. ROR icon California Institute of Technology

Description

GH9-3 ; sq3 ; 1.2s

Technical info

Tilt Series Date: 2018-10-10

Data Taken By: William Nicolas

Species / Specimen: Gluconacetobacter hansenii

Strain: ATCC 23769

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 50.0°), step: 2°, constant angular increment, dosage: 130.0 eV/Ų, defocus: -8.0 μm, magnification: 22500x.

Microscope: Caltech Polara

Acquisition Software: Undefined

Upload Method: pipeline

Processing Software Used: RAPTOR

Purification / Growth Conditions / Treatment: Overnight 6mL culture - Shaken, centrif 2500rcf 10min 30C, resuspended in 0.5mL SH medium.

Sample Preparation: +13min post-prep' - 30C, 100% hum Manual backblot 5s FM layed on grid prior and backblotted to dry excess liquid.

Attached Files

gh9-3_sq3_cell5.mrc, Tilt Series (Pixel Size 0.48 nm), 1.6 GB

gh9-3_sq3_cell5_full.rec, Reconstruction (Pixel Size 0.96 nm), 2.8 GB

Files

keyimg_wnj2018-10-10-4.jpg

Files (4.4 GB)

Name Size
1.6 GB (external) Download
2.8 GB (external) Download
md5:c2911494ab5a1befb45295f360b5984a
4.3 MB Preview Download
md5:656606b84cfa43c2b1f5068b1e8fe04c
9.4 MB Preview Download
md5:6fbce397d5e86dedb6f05ae937ff54c9
463.5 kB Preview Download
md5:80e9afc8d321beb364b237dd71f9cdd8
461.6 kB Preview Download
md5:8cdfdefa9214f486fdf9fc65e65d5b7e
461.3 kB Preview Download

Additional details

Identifiers

DOI
10.22002/w4pr2-srt16
TILT_SERIES_ID
wnj2018-10-10-4

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2018-10-10
Created
2018-10-11
Updated
2018-10-12