Published 2012 | Version v1
Dataset Open

COS7 tfx CHMP4A and VPS4B(E235Q)

  • 1. ROR icon California Institute of Technology

Contributors

Researcher:

Description

Whole cell ECT of COS7 cell expressing CHMP4A and VPS4B(E235Q)

Technical info

Tilt Series Date: 2012-03-13

Data Taken By: Cora Woodward

Species / Specimen: Cercopithecus aethiops (monkey, African green)

Strain: COS-7 ATCC CRL-1651

Tilt Series Settings: Single Axis, tilt range: (-50.0°, 50.0°), step: 1.5°, constant angular increment, dosage: 60.0 eV/Ų, defocus: -6.0 μm, magnification: 22500x.

Acquisition Software: UCSF tomo

Upload Method: rundir

Processing Software Used: Raptor

Collaborators and Roles: CHMP4A and VPS4B (E235Q) expression constructs provided by W.Sundquist (Utah)

Purification / Growth Conditions / Treatment: lipofectamine transfection

Sample Preparation: manual blotting

Attached Files

12mar13_c121_1_Cos74ADN_AT4bt1_0003.mrc, Tilt Series (Pixel Size 0.9 nm), 561.7 MB

12mar13_c121_1_Cos74ADN_AT4bt1_0003_full.rec, Reconstruction (Pixel Size 1.8 nm), 722.6 MB

Files

keyimg_cwl2012-03-13-2.jpg

Files (1.3 GB)

Name Size
561.7 MB (external) Download
722.6 MB (external) Download
md5:01ea138f3a0c85a555b02f559f5d81a7
1.2 MB Preview Download
md5:6b2c38a5c77e5fef0890f3fab0b7fb9b
23.3 MB Preview Download

Additional details

Identifiers

DOI
10.22002/w8beg-z3f15
TILT_SERIES_ID
cwl2012-03-13-2

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2012-03-13
Created
2012-03-20
Updated
2012-03-20