Bdellovibrio attack-phase
Contributors
Researchers:
Description
Technical info
Tilt Series Date: 2013-09-10
Data Taken By: Yi-Wei Chang
Species / Specimen: Bdellovibrio bacteriovorus
Strain: HD100
Tilt Series Settings: Single Axis, tilt range: (-50.0°, 50.0°), step: 2.0°, constant angular increment, dosage: 20.0 eV/Ų, defocus: -8.0 μm, magnification: 26000x.
Microscope: Janelia Titan
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Strain from Liz Sockett, Cells prepared by Lee Rettberg
Purification / Growth Conditions / Treatment: Cells grown in S17-1 prey solution at 30 degrees, OD600=0.2, filtered by 0.22 um filter, mixed with purified WM3433 mini E. coli for 10 min before freezing
Sample Preparation: Vitrobot settings: 100% humidity, 3µl sample, 10 nm BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Quantifoil Cu Finder grid
Attached Files
20130910_Janelia_Bdellovibrio_WM3433_20015.mrc, Tilt Series (Pixel Size 0.42 nm), 1.4 GB
20130910_Janelia_Bdellovibrio_WM3433_20015_full.rec, Reconstruction (Pixel Size 1.68 nm), 688.9 MB
Files
keyimg_ycw2013-09-10-44.jpg
Additional details
Identifiers
- DOI
- 10.22002/wac75-c1p82
- TILT_SERIES_ID
- ycw2013-09-10-44
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2013-09-10
- Created
-
2013-09-11
- Updated
-
2013-09-27