Published 2009
| Version v1
Dataset
Open
IST1N tubes
Contributors
Researcher:
Description
IST1N, ESCRT, tubes
Technical info
Tilt Series Date: 2009-11-11
Data Taken By: Megan Dobro
Species / Specimen: IST1N tubes
Strain:
Tilt Series Settings: Single Axis, tilt range: (60.0°, 60.0°), step: 2 degrees°, constant angular increment, dosage: 1.0 eV/Ų, defocus: 3.5 μm, magnification: 41000x.
Acquisition Software: Leginon
Upload Method: webload
Processing Software Used: Raptor
Collaborators and Roles: John McCullough purified in Utah
Purification / Growth Conditions / Treatment: dialysis step upon receiving protein
Sample Preparation: quantifoil, Vitrobot, various blot times and offset, 10 nm gold, 100 % humidity, 22 degrees
Attached Files
09nov11b_Box380grid2_005.mrc, Tilt Series (Pixel Size 0.51 nm), 520.1 MB
Files
keyimg_mdj2009-11-11-4.jpg
Additional details
Identifiers
- DOI
- 10.22002/wf1yw-0yv76
- TILT_SERIES_ID
- mdj2009-11-11-4
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2009-11-11
- Created
-
2010-01-21
- Updated
-
2010-01-21