Published 2014
| Version v1
Dataset
Open
NR1769pKM101conjNR1769
Description
Conjugation on 2% agar plate for 1h at 37C between skinny E. coli NR1769 having the F plasmid pKM101 and skinny E. coli NR1769
Technical info
Tilt Series Date: 2014-10-24
Data Taken By: Elitza Tocheva
Species / Specimen: Escherichia coli
Strain: NR1769 pKM101
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Ų, defocus: -10.0 μm, magnification: 22500x.
Microscope: Caltech Polara
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Christian Baron made the strain with the plasmid.
Sample Preparation: Vitrobot Mark IV
Attached Files
NR1760_pk101_conj0012.mrc, Tilt Series (Pixel Size 0.5 nm), 3.4 GB
NR1760_pk101_conj0012_full.rec, Reconstruction (Pixel Size 2.0 nm), 980.0 MB
Files
keyimg_etl2014-10-24-25.jpg
Additional details
Identifiers
- DOI
- 10.22002/whad4-8cd95
- TILT_SERIES_ID
- etl2014-10-24-25
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2014-10-24
- Created
-
2014-10-25
- Updated
-
2014-12-19