Sulfolobus cells synchronized for division

Description

Technical info

Tilt Series Date: 2010-12-04

Data Taken By: Megan Dobro

Species / Specimen: Sulfolobus acidocaldarius

Strain:

Tilt Series Settings: Single Axis, tilt range: (-65.0°, 65.0°), step: 1°, constant angular increment, dosage: 2.7 eV/Ų, defocus: -15.0 μm, magnification: 18000x.

Acquisition Software: Leginon

Upload Method: rundir

Processing Software Used: Raptor

Collaborators and Roles: grown and synchronized by Rachel Samson in Stephen Bell's lab, Oxford University

Purification / Growth Conditions / Treatment: grown in Brock's Medium at 80C, synchronize with Baby Machine

Sample Preparation: frozen at Kay Grunewald's lab. Harrick plasma cleaner. Blotted on both sides, 4 sec, sponge for humidity

mdj2010-12-04-2_slicer8177.jpg: pili . Sulfolobus acidocaldarius mdj2010-12-04-2_slicer8178.jpg: ring . Sulfolobus acidocaldarius mdj2010-12-04-2_slicer8180.jpg: filament . Sulfolobus acidocaldarius mdj2010-12-04-2_slicer8182.jpg: filament . Sulfolobus acidocaldarius mdj2010-12-04-2_slicer8183.jpg: pili . Sulfolobus acidocaldarius mdj2010-12-04-2_slicer8186.jpg: filament . Sulfolobus acidocaldarius

Attached Files

10dec04b_Box13grid3_002new.mrc, Tilt Series (Pixel Size 1.258 nm), 1.1 GB

10dec04b_Box13grid3_002new_full.rec, Reconstruction (Pixel Size 2.38 nm), 1.0 GB