Published 2018
| Version v1
Dataset
Open
gh2-2_sq2_cell2
Description
GH2-2, Sq2, IT 1S
Technical info
Tilt Series Date: 2018-04-30
Data Taken By: William Nicolas
Species / Specimen: Gluconacetobacter hansenii
Strain: ATCC 23769
Tilt Series Settings: Single Axis, tilt range: (-55.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Ų, defocus: -4.0 μm, magnification: 26000x.
Microscope: Caltech Titan Krios
Acquisition Software: Serial EM
Upload Method: pipeline
Processing Software Used: Raptor
Purification / Growth Conditions / Treatment: Static overnight culture (20h) - Spun down 10min - 27C - 2000rcf - resuspended in 0.5mL - put back at 30C for 4h.
Sample Preparation: Backside manual blotting 3s
Attached Files
sq2_cell2.mrc, Tilt Series (Pixel Size 0.53 nm), 3.4 GB
sq2_cell2_full.rec, Reconstruction (Pixel Size 1.06 nm), 1.4 GB
Files
keyimg_wnj2018-04-30-40.jpg
Additional details
Identifiers
- DOI
- 10.22002/wnksh-47e06
- TILT_SERIES_ID
- wnj2018-04-30-40
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2018-04-30
- Created
-
2018-05-03
- Updated
-
2018-05-18