Caulobacter crescentus ftsE del

Description

Technical info

Tilt Series Date: 2016-07-15

Data Taken By: Qing Yao

Species / Specimen: Caulobacter crescentus

Strain: NA1000

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Ų, defocus: -11.0 μm, magnification: 22500x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: UCSFtomo

Collaborators and Roles: Goley ED at Johns Hopkins University School of Medicine

Purification / Growth Conditions / Treatment: C. crescentus was from Yi-Wei's cryo-stock and was grown in PYE medium overnight at 30°c 250 rpm. In the next days' morning, 1/20 subculture into the M2G medium. When OD600 reaches 0.30, 2 ml medium was spun down gently (3000 rpm for 3 min) and the resuspension was 100 ul for cryo-grid preparation.

Sample Preparation: Glow discharged Quantifoil R2/R holey carbon film. 4 ul concentrated gold suspension was added to 16 ul bacteria and mix before plunge freezing. Plunge freezing condition: 6(force)-2(duration time)-1(dry time). Tilt Series Setting: single tilt. constant angular increment, step: 1. tilt range: (-60, 60). dosage: 180/A2. defocus: -10 um. magnification: 27500. Tilt Series Setting: single tilt. constant angular increment, step: 1.0. tilt range: (-60, 60).

Attached Files

20001.mrc, Tilt Series (Pixel Size 0.48 nm), 861.6 MB

20001_part121_56.rec, Reconstruction (Pixel Size 1.92 nm), 78.3 MB