Regeneration protocol - Drosophila

Creators: Goentoro, Lea A.¹

1. California Institute of Technology

Contact person:: Goentoro, Lea A.¹

Researchers:: Li, Yutian¹; Tan, Fayth¹; Lee, Iris¹; Sarma, Anish¹

1. California Institute of Technology

Description

This protocol details the step-by-step procedure to amputate adult fly limb; administer leucine, glutamine, and insulin to the fly; and assess regenerative response in the limb. This protocol was used in Abrams et al., 2021 (doi.org/10.1101/2020.11.21.392720).

Files

Drosophila protocol v0623.pdf

Files (13.4 MB)

Name	Size	Actions
Drosophila protocol v0623.pdf md5:96e2664583b7d7aaafb96a23905b6298	883.7 kB	Preview Download
Drosophila-protocol-v0623-Movie-1.avi md5:9d237fc56d779b8c963403d9213b7986	12.5 MB	Download

Additional details