EPEC_8770_U2OS
Contributors
Researcher:
Description
Technical info
Tilt Series Date: 2020-02-15
Data Taken By: Mohammed Kaplan
Species / Specimen: Escherichia coli
Strain: 8770, EPEC 8612 + Δbfp ΔtraD-ygeB ΔfinO-traI
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 100.0 eV/Ų, defocus: -6.0 μm, magnification: 19500x.
Microscope: Caltech Titan Krios
Acquisition Software: Serial EM
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: The strain EPEC 8770 is obtained from Ritesh (Ilan Rosenshine lab, the Hebrew university) and . Amit (Sigal ben Yehuda lab). U2OS was obtained from Wei Zhao in the Jensen lab.
Purification / Growth Conditions / Treatment: U2OS were grown on gold finders grids overnight in DMEM medium at 37 C. next day the grids were washed with PBS and incubated with DMEM+FCS and bacteria were added . (1:100) and the grids were cultured for 3.5 hours at 37C. Then the grids were washed twice with PBS and incubated with starvation buffer for 1 hour. The samples were plunge . frozen.
Sample Preparation: Samples were blotted manually.
Attached Files
EPEC_8770_U2OS_002_af.mrc, Tilt Series (Pixel Size 0.449 nm), 1.9 GB
EPEC_8770_U2OS_002_af_part41_11.rec, Reconstruction (Pixel Size 1.796 nm), 737.3 MB
Files
keyimg_mka2020-02-15-2.jpg
Additional details
Identifiers
- DOI
- 10.22002/wv0d2-80e86
- TILT_SERIES_ID
- mka2020-02-15-2
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2020-02-15
- Created
-
2020-02-16
- Updated
-
2022-10-05