Published 2010 | Version v1
Dataset Open

M222R chemoreceptor mutant

  • 1. ROR icon California Institute of Technology

Contributors

Researcher:

Description

M222R HAMP mutant locked in 'CHeA inactivating state'

Technical info

Tilt Series Date: 2010-10-08

Data Taken By: Ariane Briegel

Species / Specimen: Escherichia coli

Strain:

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -10.0 μm, magnification: 34000x.

Acquisition Software: Leginon

Upload Method: webload

Processing Software Used: IMOD

Collaborators and Roles: John Parkinson

Purification / Growth Conditions / Treatment: grown in TB medium, induced with salycilate (0.6 µM) for three hours, then penicillin treated for one hour

Attached Files

10oct08a_M222R_010.st, Tilt Series (Pixel Size 0.0 nm), 1.0 GB

10oct08a_M222R_010_full.rec, Reconstruction (Pixel Size 0.0 nm), 3.4 GB

Files

keyimg_aba2010-10-08-3.jpg

Files (4.4 GB)

Name Size
1.0 GB (external) Download
3.4 GB (external) Download
md5:6d0ed51cbc7387229202135f75b2e6f5
4.9 MB Preview Download
md5:2cba43cd381525871da0facd617317d3
8.4 MB Preview Download

Additional details

Identifiers

DOI
10.22002/wx79y-7r190
TILT_SERIES_ID
aba2010-10-08-3

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2010-10-08
Created
2010-10-19
Updated
2010-10-19