Published 2017 | Version v1
Dataset Open

T5_wt

  • 1. ROR icon California Institute of Technology

Contributors

Description

Minicells have been isolated by high-speed centrifugation and gold beads are added.

Technical info

Tilt Series Date: 2017-03-11

Data Taken By: Poorna Subramanian

Species / Specimen: Escherichia coli

Strain: EC93 stick, UPEC536

Tilt Series Settings: Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 155.0 eV/Ų, defocus: -10.0 μm, magnification: 27500x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: autoPatch

Collaborators and Roles: Zach Ruhe and Chris Hayes

Purification / Growth Conditions / Treatment: Freezing in vitrobot

Sample Preparation: The sample prepared by Taylor Steven

Attached Files

4sqs_T5_1693cysAulabelled0012.mrc, Tilt Series (Pixel Size 0.39 nm), 3.7 GB

4sqs_T5_1693cysAulabelled0012.rec, Reconstruction (Pixel Size 1.56 nm), 890.9 MB

Files

keyimg_psa2017-03-11-12.jpg

Files (4.6 GB)

Name Size
3.7 GB (external) Download
890.9 MB (external) Download
md5:499a921baf916a89319d1d7fe00009ce
1.0 MB Preview Download
md5:cc82e0814b7def6fe3bfa167d9f434bc
17.3 MB Preview Download

Additional details

Identifiers

DOI
10.22002/wxcfz-2v882
TILT_SERIES_ID
psa2017-03-11-12

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2017-03-11
Created
2017-03-12
Updated
2019-03-25