Hela-cryoFIB
Description
Technical info
Tilt Series Date: 2017-03-27
Data Taken By: Wei Zhao
Species / Specimen: HeLa cells
Strain: ATCC
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -6.0 μm, magnification: 27500x.
Microscope: Caltech Polara
Acquisition Software: SerialEM
Upload Method: reupload_downloaded
Processing Software Used: IMOD
Purification / Growth Conditions / Treatment: Hela cells grown on Au finder grids for 28-48 hours.Grids coated with PL.
Sample Preparation: Sample Preparation: Cells were plunge frozen using the vitrobot III blotted manually from back(drain time 1s).
Attached Files
TS10.mrc, Tilt Series (Pixel Size 0.372 nm), 1.7 GB
TS1_4bin_full.rec, Reconstruction (Pixel Size 0.0 nm), 32.9 MB
TS1_4bin_sirt16_flipint.rec, Reconstruction (Pixel Size 0.0 nm), 356.4 MB
Files
keyimg_wza2017-03-27-1.jpg
Additional details
Identifiers
- DOI
- 10.22002/wyvvs-frp98
- TILT_SERIES_ID
- wza2017-03-27-1
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2017-03-27
- Created
-
2018-06-21
- Updated
-
2018-06-21