Published 2011 | Version v1
Dataset Open

E.coli "strain11" A413T CheAoff

  • 1. ROR icon California Institute of Technology

Description

E.coli methylation site mutant locked in a CheA inactivating state

Technical info

Tilt Series Date: 2011-08-18

Data Taken By: Ariane Briegel

Species / Specimen: Escherichia coli

Strain:

Tilt Series Settings: Single Axis, tilt range: (-63.0°, 63.0°), step: °, constant angular increment, dosage: 160.0 eV/Ų, defocus: -10.0 μm, magnification: 34000x.

Acquisition Software: UCSF Tomo

Upload Method: webload

Processing Software Used: IMOD

Collaborators and Roles: mutant from John S. Parkinson

Purification / Growth Conditions / Treatment: cells grown at 30C in TB to log phase, then treated with penicillin for one hour

Attached Files

Ecolistrain11_2_081811_0001.st, Tilt Series (Pixel Size 0.0 nm), 1.1 GB

Ecolistrain11_2_081811_0001.rec, Reconstruction (Pixel Size 0.0 nm), 2.4 GB

Files

keyimg_aba2011-08-18-27.jpg

Files (3.4 GB)

Name Size
2.4 GB (external) Download
1.1 GB (external) Download
md5:2526862d5acd6f0b6b427d8d6f7659e5
4.6 MB Preview Download
md5:09f61100557d11050df94d6a3c71b980
13.5 MB Preview Download

Additional details

Identifiers

DOI
10.22002/x21w8-vxs57
TILT_SERIES_ID
aba2011-08-18-27

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2011-08-18
Created
2011-10-24
Updated
2011-10-24