Published 2014
| Version v1
Dataset
Open
Agro pJW58
Contributors
Researcher:
Description
wild-type Agro with plasmid-borne VirC1-GFP translational fusion under control of the virB promoter. Kanamycin-resistant (150 to 300 ug/mL)
Technical info
Tilt Series Date: 2014-03-05
Data Taken By: Elitza Tocheva
Species / Specimen: Agrobacterium tumefaciens
Strain:
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Ų, defocus: -8.0 μm, magnification: 22500x.
Microscope: Caltech Polara
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Clay Fuqua
Purification / Growth Conditions / Treatment: Cells were grown in AB medium
Sample Preparation: Vitrobot
Attached Files
4_pJW58_20008.mrc, Tilt Series (Pixel Size 0.4 nm), 3.4 GB
4_pJW58_20008_part121_14.rec, Reconstruction (Pixel Size 1.6 nm), 356.4 MB
Files
keyimg_etl2014-03-05-15.jpg
Additional details
Identifiers
- DOI
- 10.22002/x52s1-ca958
- TILT_SERIES_ID
- etl2014-03-05-15
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2014-03-05
- Created
-
2014-03-07
- Updated
-
2015-01-05