Published 2019 | Version v1
Dataset Open

bio12-2_lam2_atlas

  • 1. ROR icon California Institute of Technology

Description

. [added 2019-10-14 12:31:18] High reZ Atlas of the lamella done at 19500x mag, with preview settings to keep the dose low (0.096 exposure time)

Technical info

Tilt Series Date: 2019-09-08

Data Taken By: William Nicolas

Species / Specimen: Gluconacetobacter hansenii

Strain: ATCC 23769

Tilt Series Settings: Single Axis, tilt range: (0.0°, 0.0°), step: °, constant angular increment, dosage: 0.0 eV/Ų, defocus: 0.0 μm, magnification: 0x.

Microscope: 0

Acquisition Software: Undefined

Upload Method: webload

Processing Software Used:

Purification / Growth Conditions / Treatment: I inoculated 10 petri dishes filled with 1mL of SH with a biofilm from 12.02.19. 5 grids will be incubated 3 hours at 30C, static conditions

Sample Preparation: Quantifoil Au R2/2 NH2 200 glowdischarged 1min - 15mA. Coated with Fibronectin BT5s BF15 DT2s (frontblot only) - Nice biofilm that peeled off nicely. FIB milled grid

Attached Files

atlas_lam2.mrc, Tilt Series (Pixel Size 0.0 nm), 3.4 GB

Files

keyimg_wnj2019-09-08-27.jpg

Files (3.4 GB)

Name Size
3.4 GB (external) Download
md5:fb631055b5b161713b83c590cac48daf
9.5 MB Preview Download

Additional details

Identifiers

DOI
10.22002/x64hq-8z738
TILT_SERIES_ID
wnj2019-09-08-27

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2019-09-08
Created
2019-10-14
Updated
2022-11-16