Pyruvate dehydrogenase

Description

Technical info

Tilt Series Date: 2004-08-24

Data Taken By: Gavin Murphy

Species / Specimen: Escherichia coli

Strain: purified pyruvate dehydrogenase

Tilt Series Settings: Dual Axis, tilt range: (-63.0°, 63.0°), step: 3°, constant angular increment, dosage: 120.0 eV/Ų, defocus: -8.0 μm, magnification: 0x.

Acquisition Software: UCSF Tomo

Upload Method: rundir

Processing Software Used: imod, bsoft

Collaborators and Roles: Protein provided by Terrence Wagenknecht, Wadsworth, Albany, NY; Data collected and processed by Gavin Murphy

Purification / Growth Conditions / Treatment: E. coli. Pyruvate dehydrogenase and 2-oxoglutarate dehydrogenase obtained from Terrence Wagenknecht at Wadsworth, Albany, NY. Fortunately, I didn't have to purify anything. Protein was diluted to I think 8 mg/ml and applied to grids.

Sample Preparation: quantifoil grid, 10nm gold, 100% humidity...

Attached Files

P3a_S46G3_60e10d27kx.mrc, Tilt Series (Pixel Size 0.82 nm), 369.2 MB