Published 2015
| Version v1
Dataset
Open
pED208_pilated
Description
E coli strain with plasmid pED208 overexpresses T4SS and F-like pili
Technical info
Tilt Series Date: 2015-01-14
Data Taken By: Elitza Tocheva
Species / Specimen: Escherichia coli
Strain:
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 180.0 eV/Ų, defocus: -10.0 μm, magnification: 22500x.
Microscope: Caltech Polara
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Cells were obtained from Laura Frost
Purification / Growth Conditions / Treatment: o/n liquid LB
Sample Preparation: Vitrobot
Attached Files
pED208b0035.mrc, Tilt Series (Pixel Size 0.5 nm), 3.4 GB
pED208b0035_full.rec, Reconstruction (Pixel Size 2.0 nm), 445.4 MB
Files
keyimg_etl2015-01-14-89.jpg
Additional details
Identifiers
- DOI
- 10.22002/x9v01-wf321
- TILT_SERIES_ID
- etl2015-01-14-89
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2015-01-14
- Created
-
2015-01-15
- Updated
-
2015-01-16