gh2-4_sq1_cell2
Description
Technical info
Tilt Series Date: 2018-05-16
Data Taken By: William Nicolas
Species / Specimen: Gluconacetobacter hansenii
Strain: ATCC 23769
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 181.0 eV/Ų, defocus: -4.0 μm, magnification: 33000x.
Microscope: Caltech Titan Krios
Acquisition Software: Serial EM
Upload Method: pipeline
Processing Software Used: Raptor
Purification / Growth Conditions / Treatment: Static overnight culture (20h) - Spun down 10min - 27C - 2000rcf - resuspended in 0.5mL - put back at 30C for 4h
Sample Preparation: Backside manual blotting 5s
wnj2018-05-16-13_slicer19516.jpg: Cortical sheet again
Attached Files
gh2-4_sq1_cell2.mrc, Tilt Series (Pixel Size 0.43 nm), 1.7 GB
gh2-4_sq1_cell2_full.rec, Reconstruction (Pixel Size 0.86 nm), 2.1 GB
Files
keyimg_wnj2018-05-16-13.jpg
Additional details
Identifiers
- DOI
- 10.22002/xfwan-8bj32
- TILT_SERIES_ID
- wnj2018-05-16-13
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2018-05-16
- Created
-
2018-05-19
- Updated
-
2018-11-06