Caltech Tomography Archive
Published 2014 | Version v1
Dataset Open

Myxococcus xanthus delta-tsaP

Chang, Yi-Wei1
  • 1. ROR icon California Institute of Technology
Researcher:
Sogaard-Andersen, Lotte

Description

Grid box YWC106

Files

keyimg_ycw2014-07-07-52.jpg
Files (18.3 MB)
Name Size
keymov_ycw2014-07-07-52.mp4
md5:71d41987e148641823b26f9e138ca338
17.2 MB Download
keyimg_ycw2014-07-07-52.jpg
md5:09c284833502dfac62c985052131f3da
1.1 MB Preview Download

Technical info

Tilt Series Date: 2014-07-07

Data Taken By: Yi-Wei Chang

Species / Specimen: Myxococcus xanthus

Strain:

Tilt Series Settings: Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 200.0 eV/Ų, defocus: -8.0 μm, magnification: 27500x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Strain form Lotte Sogaard-Andersen

Purification / Growth Conditions / Treatment: Cells grown 48 hr in shaking CTT medium at 30 degrees without antibiotics. OD600=0.7

Sample Preparation: Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. Manual blot, ~3 sec blot time. Ethane/propane mix. Grid: Quantifoil Cu 2/2 Finder.

External Files

Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/ycw2014-07-07-52

20140707_YWC106_30003.mrc, Tilt Series (Pixel Size 0.39 nm), 3.7 GB Download

20140707_YWC106_30003_part131_64.rec, Reconstruction (Pixel Size 1.56 nm), 712.7 MB Download

Additional details

Identifiers Funding Dates
Created:
May 24, 2023
Modified:
May 24, 2023