C. crescentus NA1000 Tad pilus
Description
Technical info
Tilt Series Date: 2019-08-17
Data Taken By: Steven Wang
Species / Specimen: Caulobacter crescentus
Strain: NA1000
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 100.0 eV/Ų, defocus: -6.0 μm, magnification: 26000x.
Microscope: Caltech Titan Krios
Acquisition Software: Serial EM
Upload Method: pipeline
Processing Software Used: None
Collaborators and Roles: Patrick Viollier and Lujia Zhang
Purification / Growth Conditions / Treatment: Swarmer cells purified using Percoll (colloidal silica). Cultured using M2G media.
Sample Preparation: 10 nm gold beads. Blotting force 4, blotting time 4, draining time 1. Quantifoil copper grids.
Attached Files
caulobacter_na1000_wt_185_af.mrc, Tilt Series (Pixel Size 0.336 nm), 3.9 GB
caulobacter_na1000_wt_185_af.mrc.mdoc, 802.0 B
Files
keyimg_swy2019-08-17-183.jpg
Additional details
Identifiers
- DOI
- 10.22002/xp4dk-g0496
- TILT_SERIES_ID
- swy2019-08-17-183
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2019-08-17
- Created
-
2019-08-29
- Updated
-
2020-01-02