HeLa CHMP4A+VPS4DN
Description
Technical info
Tilt Series Date: 2011-07-24
Data Taken By: Cora Woodward
Species / Specimen: HeLa cells
Strain: ATCC
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -7.0 μm, magnification: 22500x.
Acquisition Software: UCSF tomo
Upload Method: rundir
Processing Software Used: imod
Collaborators and Roles: CHMP4A and VPS4DN obstruct provided by Wes Sundquist. Cells transfected, frozen and imaged at Caltech by Cora Woodward
Purification / Growth Conditions / Treatment: whole cells grown on grids and transfected
Sample Preparation: manual blotting
Attached Files
11jul24_hela_4aDNlipo_12_trim_full.rec, Reconstruction (Pixel Size 2.0 nm), 103.2 MB
11jul24_hela_4aDNlipo_12_trim.st, Tilt Series (Pixel Size 0.5 nm), 743.2 MB
Files
keyimg_cwl2011-07-24-3.jpg
Additional details
Identifiers
- DOI
- 10.22002/xp9wh-zhr88
- TILT_SERIES_ID
- cwl2011-07-24-3
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2011-07-24
- Created
-
2015-09-16
- Updated
-
2015-09-16