Published 2010 | Version v1
Dataset Open

MamJ deletion w/o iron

Creators

  • 1. ROR icon California Institute of Technology

Contributors

Description

No magnetosome vesicles, suspected as a mutant that has lost the magnetosome island

Technical info

Tilt Series Date: 2010-01-22

Data Taken By: Zhuo Li

Species / Specimen: Magnetospirillum magneticum

Strain: AMB-1

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 120.0 eV/Ų, defocus: -16.0 μm, magnification: 18000x.

Acquisition Software: Leginon

Upload Method: webload

Processing Software Used: Raptor and Peach, IMOD

Collaborators and Roles: Mutant constructed by Arash Komeili, cells shipped to and imaged by Zhuo Li

Purification / Growth Conditions / Treatment: Stationary phase

Sample Preparation: Quantifoid grid, 10-nm gold, 100% humidity

Attached Files

10jan22a_dJnofe2_005.mrc, Tilt Series (Pixel Size 0.0 nm), 1.0 GB

10jan22a_dJnofe2_005_part.rec, Reconstruction (Pixel Size 0.0 nm), 524.3 MB

10jan22a_dJnofe2_0051K.rec, Reconstruction (Pixel Size 0.0 nm), 100.2 MB

Files

keyimg_zla2010-01-22-2.jpg

Files (1.7 GB)

Name Size
1.0 GB (external) Download
100.2 MB (external) Download
524.3 MB (external) Download
md5:468f11d27db1f34e3b29a9571dfd961e
424.6 kB Preview Download
md5:e5a3e1e5715ff7c5ed378be138942ab5
7.2 MB Preview Download

Additional details

Identifiers

DOI
10.22002/xrfa5-03m63
TILT_SERIES_ID
zla2010-01-22-2

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2010-01-22
Created
2010-02-12
Updated
2010-02-12