Dividing Caulobacter
Description
Technical info
Tilt Series Date: 2016-02-28
Data Taken By: Qing Yao
Species / Specimen: Caulobacter crescentus
Strain: NA1000
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 180.0 eV/Ų, defocus: -11.0 μm, magnification: 22500x.
Microscope: Caltech Polara
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Purification / Growth Conditions / Treatment: Glow discharged Quantifoil R2/R holey carbon film. 4 ul concentrated gold suspension was added to 16 ul bacteria and mix before plunge freezing. Plunge freezing condition: 6(force)-2(duration time)-1(dry time). Tilt Series Setting: single tilt. constant angular increment, step: 1. tilt range: (-60, 60). dosage: 180/A2. defocus: -10 um. magnification: 27500.
Sample Preparation: single tilt. constant angular increment, step: 1.0. tilt range: (-60, 60). dosage: 180/A2. defocus: -10um. magnification: 27500.
Attached Files
view80023_full.rec, Reconstruction (Pixel Size 1.92 nm), 445.4 MB
view80023.mrc, Tilt Series (Pixel Size 0.48 nm), 3.4 GB
Files
keyimg_qya2016-02-28-23.jpg
Additional details
Identifiers
- DOI
- 10.22002/xt5n3-6wx77
- TILT_SERIES_ID
- qya2016-02-28-23
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2016-02-28
- Created
-
2016-02-29
- Updated
-
2022-10-12