COS7 tfx CHMP4A and VPS4B(E235Q)
Contributors
Researcher:
Description
Technical info
Tilt Series Date: 2012-03-14
Data Taken By: Cora Woodward
Species / Specimen: Cercopithecus aethiops (monkey, African green)
Strain: COS-7 ATCC CRL-1651
Tilt Series Settings: Single Axis, tilt range: (-50.0°, 50.0°), step: 1.5°, constant angular increment, dosage: 60.0 eV/Ų, defocus: -6.0 μm, magnification: 22500x.
Acquisition Software: UCSF tomo
Upload Method: rundir
Processing Software Used: Raptor
Collaborators and Roles: CHMP4A and VPS4B (E235Q) expression constructs provided by W.Sundquist (Utah)
Purification / Growth Conditions / Treatment: lipofectamine transfection
Sample Preparation: manual blotting
Attached Files
12mar14_c121_1_Cos74ADN_AT1bt10006.mrc, Tilt Series (Pixel Size 0.9 nm), 561.7 MB
12mar14_c121_1_Cos74ADN_AT1bt10006_part68_4.rec, Reconstruction (Pixel Size 1.8 nm), 722.6 MB
Files
keyimg_cwl2012-03-14-21.jpg
Additional details
Identifiers
- DOI
- 10.22002/xte3j-9d657
- TILT_SERIES_ID
- cwl2012-03-14-21
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2012-03-14
- Created
-
2012-03-20
- Updated
-
2012-03-20