E. coli CusCFBA-CusC-K98H
Contributors
Researcher:
Description
Technical info
Tilt Series Date: 2013-06-07
Data Taken By: Yi-Wei Chang
Species / Specimen: Escherichia coli
Strain:
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -8.0 μm, magnification: 22500x.
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Strain from Edward Yu
Purification / Growth Conditions / Treatment: Cells grown in LB medium + 100 ug/ml Amp at 37 degrees to OD600=0.6. Add final 0.2% L-arabinose, shake at 20 C for 16 hr
Sample Preparation: Vitrobot settings: 100% humidity, 3µl sample, 10 mg/ml BSA treated gold. -3 offset, 4 sec blot time, no drain. Ethane/propane mix. Grid: Quantifoil Cu 200 2/2.
Attached Files
CusCFBA_CusCK98H_20016.mrc, Tilt Series (Pixel Size 0.48 nm), 3.4 GB
CusCFBA_CusCK98H_20016_part121_38.rec, Reconstruction (Pixel Size 1.92 nm), 712.7 MB
Files
keyimg_ycw2013-06-07-19.jpg
Additional details
Identifiers
- DOI
- 10.22002/xwsn6-zdy70
- TILT_SERIES_ID
- ycw2013-06-07-19
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2013-06-07
- Created
-
2013-06-08
- Updated
-
2013-07-25