Published 2020 | Version v1
Dataset Open

BacillusSubtilis_328_4hr

  • 1. ROR icon California Institute of Technology

Description

Bacillus subtitles strain 328 overexpressing ymdB.

Technical info

Tilt Series Date: 2020-02-26

Data Taken By: Mohammed Kaplan

Species / Specimen: Bacillus subtilis

Strain: AB328, ΔponA ΔymdB amyE::ymdB Δhag

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -6.0 μm, magnification: 19500x.

Microscope: Caltech Titan Krios

Acquisition Software: Serial EM

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Samples were prepared with Ritesh and Amit from Ilan Rosenshine and Sigal ben Yehuda labs at . the Hebrew university. See tomograms mka2020-02-08-x.

Purification / Growth Conditions / Treatment: Samples were prepared as mentioned in Bacillus subtitles tomograms taken on 8th of February . 2020.

Sample Preparation: Samples were prepared as mentioned in Bacillus subtitles tomograms taken on 8th of February . 2020. See tomograms mka2020-02-08-x.

Attached Files

Bsubtilis_328_009.mrc, Tilt Series (Pixel Size 0.449 nm), 1.9 GB

Bsubtilis_328_009_full.rec, Reconstruction (Pixel Size 1.796 nm), 737.3 MB

Files

keyimg_mka2020-02-26-20.jpg

Files (2.7 GB)

Name Size
1.9 GB (external) Download
737.3 MB (external) Download
md5:ba1fcb4fdae44b5dde21d9aa05034a6c
1.7 MB Preview Download
md5:62a53775fb30331859102ec9dde6cf06
5.3 MB Preview Download

Additional details

Identifiers

DOI
10.22002/xyfqb-tm080
TILT_SERIES_ID
mka2020-02-26-20

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2020-02-26
Created
2020-02-28
Updated
2022-10-05