Published 2017
| Version v1
Dataset
Open
T5_1693Cys_Aumalemaide_5nm
Contributors
Researchers:
Description
This is a sample in which N terminus residue 1693 has been mutated to Cys residue. Minicells have been isolated by high-speed centrifugation and cells are Au maleimide labeled. No fiducials added.
Technical info
Tilt Series Date: 2017-04-02
Data Taken By: Poorna Subramanian
Species / Specimen: Escherichia coli
Strain: EC93 stick, UPEC536
Tilt Series Settings: Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 140.0 eV/Ų, defocus: -10.0 μm, magnification: 27500x.
Microscope: Caltech Polara
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Zach Ruhe and Chris Hayes
Purification / Growth Conditions / Treatment: Freezing in vitrobot.
Sample Preparation: Sample prepared by Taylor Steven
Attached Files
cys1693_sq48_4sqs0020.mrc, Tilt Series (Pixel Size 0.39 nm), 3.7 GB
cys1693_sq48_4sqs0020_full.rec, Reconstruction (Pixel Size 1.56 nm), 445.4 MB
Files
keyimg_psa2017-04-02-42.jpg
Additional details
Identifiers
- DOI
- 10.22002/xzdnt-6ek15
- TILT_SERIES_ID
- psa2017-04-02-42
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2017-04-02
- Created
-
2017-04-04
- Updated
-
2019-03-25