Published 2007 | Version v1
Dataset Open

Rhodopseudomonas palustrus

Creators

  • 1. ROR icon California Institute of Technology

Contributors

Description

Fe-metabolizing bacterium that makes parallel membrane sheets for photosynthesis. Membrane topology is the primary interest.Keywords: photosynthetic membrane sheets, parallel membranes

Technical info

Tilt Series Date: 2007-06-22

Data Taken By: Bill Tivol

Species / Specimen: Rhodopseudomonas palustris

Strain: None

Tilt Series Settings: Single Axis, tilt range: (-59.0°, 59.0°), step: 1 deg°, constant angular increment, dosage: 200.0 eV/Ų, defocus: -10.0 μm, magnification: 0x.

Acquisition Software: UCSF Tomo

Upload Method: webload

Processing Software Used: IMOD

Collaborators and Roles: Diane Newman, PI, Lars Dietrich, Postdoc, Lars gave me the specimens

Purification / Growth Conditions / Treatment: Grown in FEM medium +10% acetate at 30 deg C in bright light.

Sample Preparation: quantifoil, 10 nm gold, 100% humidity

btf2007-06-22-6_slicer5168.jpg: Unknown btf2007-06-22-6_slicer5169.jpg: Unknown filament btf2007-06-22-6_slicer5192.jpg: Unknown btf2007-06-22-6_slicer5193.jpg: Unknown btf2007-06-22-6_slicer5197.jpg: Unknown Filament btf2007-06-22-6_slicer5199.jpg: Unknown Filament btf2007-06-22-6_slicer5203.jpg: Unknown filament btf2007-06-22-6_slicer5206.jpg: unknown filament btf2007-06-22-6_slicer5209.jpg: Unknown filaments btf2007-06-22-6_slicer5211.jpg: Unknown parallel filament btf2007-06-22-6_slicer5216.jpg: Unknown filament (parallel row) btf2007-06-22-6_slicer5217.jpg: Unknown filament (parallel row) btf2007-06-22-6_slicer5220.jpg: Unknown ring

Attached Files

Rp22Jun07_06.mrc, Tilt Series (Pixel Size 0.625 nm), 990.0 MB

Rp22Jun07_06_full.rec, Reconstruction (Pixel Size 1.25 nm), 1.3 GB

Rp22Jun07_06.rec, Reconstruction (Pixel Size 1.25 nm), 171.0 MB

Files

btf2007-06-22-6_slicer5168.jpg

Files (2.4 GB)

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990.0 MB (external) Download
171.0 MB (external) Download
1.3 GB (external) Download

Additional details

Identifiers

DOI
10.22002/y4j7h-g8c39
TILT_SERIES_ID
btf2007-06-22-6

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2007-06-22
Created
2008-06-20
Updated
2014-07-08