Caltech Tomography Archive
Published 2015 | Version v1
Dataset Open

perfusion_deltacrp

Creators

  • 1. ROR icon California Institute of Technology

Contributors

Researcher:

Description

This is a sample from the perfusion set up. This is a mutant delta crp which is supposed to make excessive nanowires and has no cytochromes.Every cell imaged was corelated to its FLM image showing a nanowire

Technical info

Tilt Series Date: 2015-07-22

Data Taken By: Poorna Subramanian

Species / Specimen: Shewanella oneidensis

Strain: MR-1

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -10.0 μm, magnification: 22500x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Batchruntomo

Collaborators and Roles: moh and sahand

Purification / Growth Conditions / Treatment: Gold grids glow dischsrged

Sample Preparation: The sample was manually plunge frozen. Gold side was glow discharged. Sample was grown on the glow discharged side, and blotted from opposite side before freezing.

Attached Files

perfusiondcrp_p1grid1_atlas10002.mrc, Tilt Series (Pixel Size 0.48 nm), 3.4 GB

perfusiondcrp_p1grid1_atlas10002.rec, Reconstruction (Pixel Size 1.92 nm), 889.0 MB

Files

keyimg_psa2015-07-22-2.jpg

Files (4.4 GB)

Name Size
md5:398146d8156e6b7f466357300097deff
934.4 kB Preview Download
md5:0d0cf8e3f4b47129ba19c3af1db4dbf6
17.0 MB Preview Download
3.4 GB (external) Download
889.0 MB (external) Download

Additional details

Identifiers

DOI
10.22002/y4mx2-qn282
TILT_SERIES_ID
psa2015-07-22-2

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2015-07-22
Created
2015-07-23
Updated
2015-08-19