Published 2011
| Version v1
Dataset
Open
Sulfolobus cells synchronized for division
- Researchers:
- Samson, Rachel
- Bell, Stephen
- University, Oxford
Citation
APA
Dobro, M. (2011). Sulfolobus cells synchronized for division [Data set]. CaltechDATA. https://doi.org/10.22002/y7m3x-7qc07
Description
search for ESCRT in dividing cells
Files
keyimg_mdj2011-01-15-4.jpg
Technical info
Tilt Series Date: 2011-01-15
Data Taken By: Megan Dobro Species / Specimen: Sulfolobus acidocaldarius Strain: Tilt Series Settings: Single Axis, tilt range: (-65.0°, 65.0°), step: 1°, constant angular increment, dosage: 1.5 eV/Ų, defocus: -15.0 μm, magnification: 18000x. Acquisition Software: Leginon Upload Method: rundir Processing Software Used: Raptor Collaborators and Roles: grown and synchronized by Rachel Samson in Stephen Bell's lab, Oxford University Purification / Growth Conditions / Treatment: grown in Brock's Medium at 80C, synchronize with Baby Machine Sample Preparation: frozen at Kay Grunewald's lab. Harrick plasma cleaner. Blotted on both sides, 6 sec, sponge for humidity mdj2011-01-15-4_slicer8672.jpg: ring . Sulfolobus acidocaldarius mdj2011-01-15-4_slicer8673.jpg: filament . Sulfolobus acidocaldariusExternal Files
Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/mdj2011-01-15-4
11jan15b_Bx8g4_005.mrc, Tilt Series (Pixel Size 1.192 nm), 1.1 GB Download 11jan15b_Bx8g4_005_part.rec, Reconstruction (Pixel Size 2.5 nm), 943.7 MB DownloadAdditional details
- TILT_SERIES_ID
- mdj2011-01-15-4
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
- Collected
-
2011-01-15
- Created
-
2012-07-09
- Updated
-
2014-07-28