H.pylori 26695 delta-cagH / AGS

Description

Technical info

Tilt Series Date: 2014-04-14

Data Taken By: Yi-Wei Chang

Species / Specimen: Helicobacter pylori

Strain: 26695

Tilt Series Settings: Single Axis, tilt range: (-65.0°, 65.0°), step: 1.0°, constant angular increment, dosage: 170.0 eV/Ų, defocus: -10.0 μm, magnification: 22500x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: H.pylori cells from Carrie Shaffer

Purification / Growth Conditions / Treatment: H.pylori cells grown in BLBB + 5% heat inactivated fetal bovine serum at 37 degrees in 5% CO2 chamber for O/N, OD600=~0.7. Back dilute to OD 0.3, grew to OD 0.6 for infection. AGS cells cultivated on QF Au finder grid for 1 day in RPMI + 10% FBS. Infect by pylori for 4 hr.

Sample Preparation: Vitrobot settings: 100% humidity, 37 C, 3µl BSA treated 20 nm gold (100 ul gold+ 25 ul BSA, pellets from 8 tubes merged into ~40 ul final solution). Manual blot by Whatman 40 filter paper for 2 sec. Ethane/propane mix.

ycw2014-04-14-16_slicer6766.jpg: filament ycw2014-04-14-16_slicer6768.jpg: peptidoglycan ycw2014-04-14-16_slicer6769.jpg: filament

Attached Files

20140414_YWC100_30005.mrc, Tilt Series (Pixel Size 0.48 nm), 3.7 GB

20140414_YWC100_30005_full.rec, Reconstruction (Pixel Size 1.92 nm), 712.7 MB