Saccharomyces cerevisiae - JAWY1
Description
Technical info
Tilt Series Date: 2016-12-12
Data Taken By: Shrawan kumar Mageswaran
Species / Specimen: Unspecified
Strain: None
Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: -100.0 eV/Ų, defocus: -8.0 μm, magnification: 27500x.
Microscope: Caltech Polara
Acquisition Software: SerialEM
Upload Method: reupload_downloaded
Processing Software Used: IMOD
Collaborators and Roles: Obtained from Dr. Greg Odorizzi @ Univ of Colorado at Boulder.
Purification / Growth Conditions / Treatment: Cells were grown overnight, diluted to 0.2 OD in the morning and allowed to grow till OD ~0.6. Heat shocked @38 deg C for 1hr50min and recovered @28deg C for 20 min (except sm2016-12-12-3 alias TS16 which was heat shocked for 1.5 hr and recovered for 40 min)
Attached Files
TS1.mrc, Tilt Series (Pixel Size 0.39 nm), 1.7 GB
TS1_4bin_NS_DB.rec, Reconstruction (Pixel Size 0.0 nm), 261.4 MB
Files
keyimg_smk2016-12-12-4.jpg
Files
(2.0 GB)
| Name | Size | |
|---|---|---|
|
md5:c1356981bd38db540607c9e64dce9535
|
1.1 MB | Preview Download |
|
md5:0965c9d37500202ce6ab310bb246dbd3
|
9.3 MB | Preview Download |
| 1.7 GB | (external) Download | |
| 261.4 MB | (external) Download | |
|
md5:ae6ce1e418f9056f6e9d2e4f3535f685
|
369.5 kB | Preview Download |
|
md5:bfaf35ad5641769fabc229dc4b0e739a
|
401.3 kB | Preview Download |
Additional details
Identifiers
- DOI
- 10.22002/yazbh-8hf68
- TILT_SERIES_ID
- smk2016-12-12-4
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2016-12-12
- Created
-
2017-01-11
- Updated
-
2017-01-17