gh2-2_sq1

Description

GH2-2, sq1, IT 1s

Files

wnj2018-04-30-34_slicer19484.jpg

Files (9.3 MB)

Name	Size	Actions
wnj2018-04-30-34_slicer19484.jpg md5:332f05804049cd429488184539bdbd19	184.2 kB	Preview Download
keyimg_wnj2018-04-30-34.jpg md5:d557a39bd45f4b533b036bca6aa6ee0a	4.3 MB	Preview Download
wnj2018-04-30-34_slicer19486.jpg md5:4889eb20210ddfb3e44f51ce9f44f5e7	296.3 kB	Preview Download
wnj2018-04-30-34_slicer19485.jpg md5:8eff890617abef6632b5f272480740c5	313.6 kB	Preview Download
wnj2018-04-30-34_slicer19487.jpg md5:4f62629fc02a930711ef201d1031cd62	285.3 kB	Preview Download
wnj2018-04-30-34_slicer19488.jpg md5:887966093c4e063e80db4ef9849d6bd3	281.7 kB	Preview Download
keymov_wnj2018-04-30-34.mp4 md5:3e408f2e85954f1229f7e05a8c43cfd5	3.6 MB	Download

Technical info

Tilt Series Date: 2018-04-30

Data Taken By: William Nicolas

Species / Specimen: Gluconacetobacter hansenii

Strain: ATCC 23769

Tilt Series Settings: Single Axis, tilt range: (-40.0°, 60.0°), step: 1°, constant angular increment, dosage: 147.0 eV/Å², defocus: -4.0 μm, magnification: 26000x.

Microscope: Caltech Titan Krios

Acquisition Software: Serial EM

Upload Method: pipeline

Processing Software Used: Raptor

Purification / Growth Conditions / Treatment: Static overnight culture (20h) - Spun down 10min - 27C - 2000rcf - resuspended in 0.5mL - put back at 30C for 4h.

Sample Preparation: Backside manual blotting 3s

wnj2018-04-30-34_slicer19484.jpg: Purple arrow : fiber with density below in peri . . Blue arrow : nascent fiber with maybe something below wnj2018-04-30-34_slicer19485.jpg: Big density in peri wnj2018-04-30-34_slicer19486.jpg: Aligned in a row? wnj2018-04-30-34_slicer19487.jpg: Opposite membrane has a peri density with this little loop right above, at the surface of OM wnj2018-04-30-34_slicer19488.jpg: Opposite membrane has a peri density with this little loop right above, at the surface of OM

External Files

Files available via S3 at https://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/wnj2018-04-30-34/rawdata/

sq1_cell2.mrc, Tilt Series (Pixel Size 0.53 nm), 2.9 GB Download

sq1_cell2_full.rec, Reconstruction (Pixel Size 1.06 nm), 1.4 GB Download

gh2-2_sq1_cell2

Description

Files

Technical info

External Files

Additional details