Lp02-fixed

Description

Technical info

Tilt Series Date: 2017-11-21

Data Taken By: Debnath Ghosal

Species / Specimen: Legionella pneumophila

Strain: Philadelphia-1_Lp02

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.5°, constant angular increment, dosage: 100.0 eV/Ų, defocus: -7.0 μm, magnification: 27500x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Joelle Gabay and Howard Shuman prepared samples, Debnath Ghosal collected data.

Purification / Growth Conditions / Treatment: Shuman Lab

Sample Preparation: 6 grids were frozen, cell density was increased to 2x by centrifugation. Vitrobot (MK iv) settings: 100% humidity, 4µl sample, Blot force 6, blot time 4.5 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.

Attached Files

Legionella_fixed_20171121a0065.mrc, Tilt Series (Pixel Size 0.39 nm), 1.7 GB

Legionella_fixed_20171121a0065.rec, Reconstruction (Pixel Size 1.56 nm), 889.0 MB