Published 2019 | Version v1
Dataset Open

Conjugation_Ecoli

  • 1. ROR icon California Institute of Technology
Researcher:
B Lindner, Ariel

Description

These are conjugation in Ecoli samples. The cells are the ANB35 cells (donor) and ANB24 . cells (recipient), More information on these strains can be found in the paper (Direct . visualization of horizontal gene transfer). These are tomograms from (square 3) in Grid 1 . from box 4 (See William's notes for more information). There are cryoLM data for this grid . obtained during the Linkam stage demo.

Files

keyimg_mka2019-06-23-31.jpg
Files (9.0 MB)
Name Size
md5:a58df66098bdf29583f8a853052902d0
7.2 MB Download
md5:82aa4c459886e19c4ac5a1c1d69ee726
1.7 MB Preview Download

Technical info

Tilt Series Date: 2019-06-23

Data Taken By: Mohammed Kaplan

Species / Specimen: Escherichia coli

Strain: ANB35, Hfr3000 mrfp1

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 110.0 eV/Ų, defocus: -8.0 μm, magnification: 26000x.

Microscope: Caltech Titan Krios

Acquisition Software: Serial EM

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: The samples were sent by Ariel B Lindner (who is the second author on the above-mentioned . paper) in Paris.

External Files

Files available via S3 at ttps://sdsc.osn.xsede.org/ini210004tommorrell/tomography_archive/mka2019-06-23-31/rawdata/

SquThreeTS.mrc, Tilt Series (Pixel Size 0.449 nm), 2.9 GB Download

SquThreeTS_full.rec, Reconstruction (Pixel Size 1.796 nm), 737.3 MB Download

SquThreeTS.mrc.mdoc, 32.2 kB Download

Additional details

Created:
June 5, 2023
Modified:
August 24, 2025