JV9298
Description
Technical info
Tilt Series Date: 2020-04-23
Data Taken By: Yuxi Liu
Species / Specimen: Legionella pneumophila
Strain: Philadelphia-1_Lp02
Tilt Series Settings: Single Axis, tilt range: (-54.0°, 54.0°), step: 3°, constant angular increment, dosage: 100.0 eV/Ų, defocus: -4.0 μm, magnification: 33000x.
Acquisition Software: Serial EM
Upload Method: rundir
Processing Software Used: imod, tomo3d
Collaborators and Roles: Sample preparation & data collection: Yuxi Liu Data processing: Phyllis Wang & Yuxi Liu
Purification / Growth Conditions / Treatment: O.D. 2.5-3.0 in AYE
Sample Preparation: Quantifoil R2/2 copper grid, 10nm gold, 100% humidity, 25deg C, 4uL of 2X concentrated cells Blotting: force 6, blot 3sec, wait 1sec
Attached Files
JV9298_PD-1-3_333.mrc, Tilt Series (Pixel Size 0.26 nm), 942.8 MB
Files
keyimg_ylx2020-04-23-361.jpg
Additional details
Identifiers
- DOI
- 10.22002/yw9m8-var20
- TILT_SERIES_ID
- ylx2020-04-23-361
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2020-04-23
- Created
-
2021-07-26
- Updated
-
2021-07-26