Published 2015 | Version v1
Dataset Open

Pseudomonas aer2 in E.coli

  • 1. ROR icon California Institute of Technology

Contributors

Researcher:

Description

Aer2 expressed from pProEx plasmid = amp resistant, in E. coli BT3388

Technical info

Tilt Series Date: 2015-03-13

Data Taken By: Ariane Briegel

Species / Specimen: Escherichia coli

Strain:

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Ų, defocus: 8.0 μm, magnification: 34000x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Kylie Watts, Loma Linda University

Purification / Growth Conditions / Treatment: grown with 0.5 ml of 0.1% thiamine per L of LBAmp

Attached Files

Aer2inEcoliTomo0005.mrc, Tilt Series (Pixel Size 0.32 nm), 3.4 GB

Aer2inEcoliTomo0005_full.rec, Reconstruction (Pixel Size 1.28 nm), 712.7 MB

Files

keyimg_aba2015-03-13-5.jpg

Files (4.2 GB)

Name Size
3.4 GB (external) Download
712.7 MB (external) Download
md5:f4fa43952d461d0c6c2930c6cebbd2f1
1.1 MB Preview Download
md5:35465485148a9e2ebe179a89e5f20779
24.9 MB Preview Download

Additional details

Identifiers

DOI
10.22002/ywtxd-j8m49
TILT_SERIES_ID
aba2015-03-13-5

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2015-03-13
Created
2015-03-13
Updated
2015-09-21