Published 2012
| Version v1
Dataset
Open
A. longum ProteinaseK digest
Contributors
Researcher:
Description
A. longum's cell wall was digested with proteinase K to determine the periplasmic layer composition
Technical info
Tilt Series Date: 2012-04-26
Data Taken By: Elitza Tocheva
Species / Specimen: Acetonema longum
Strain: None
Tilt Series Settings: Single Axis, tilt range: (-50.0°, 50.0°), step: 1°, constant angular increment, dosage: 200.0 eV/Ų, defocus: -10.0 μm, magnification: 22500x.
Microscope: Caltech Polara
Acquisition Software: UCSFTomo
Upload Method: pipeline
Processing Software Used: Raptor
Collaborators and Roles: Wesley Chen
Purification / Growth Conditions / Treatment: Cells were grown anaerobically and digested with 5mg/ml proteinase K enzyme
Sample Preparation: Vitrobot
Attached Files
alongprotk0014.mrc, Tilt Series (Pixel Size 1.9 nm), 842.5 MB
alongprotk0014_full.rec, Reconstruction (Pixel Size 3.8 nm), 619.4 MB
Files
keyimg_etl2012-04-26-14.jpg
Additional details
Identifiers
- DOI
- 10.22002/yx6tw-h1h82
- TILT_SERIES_ID
- etl2012-04-26-14
Funding
- NIH
- HHMI
- Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- John Templeton Foundation
Dates
- Collected
-
2012-04-26
- Created
-
2012-04-26
- Updated
-
2014-12-19