Published 2015 | Version v1
Dataset Open

Starving E-coli (cbpA- knockout)

  • 1. ROR icon California Institute of Technology

Contributors

Description

keywords: late stage stationary phase, starvation, MC105 cbpA-

Technical info

Tilt Series Date: 2015-08-12

Data Taken By: Ariane Briegel

Species / Specimen: Escherichia coli

Strain:

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Ų, defocus: -8.0 μm, magnification: 27500x.

Microscope: Caltech Polara

Acquisition Software: Undefined

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Strains prepared by James Haycocks in the David Grainger Lab

Purification / Growth Conditions / Treatment: Grown in LB broth at 33 degrees while shaken at 400 rpm for 11 days

Sample Preparation: manual blotting

Attached Files

cbpAminustomogramAugust0001_part121_23.rec, Reconstruction (Pixel Size 1.56 nm), 712.7 MB

cbpAminustomogramAugust0001.mrc, Tilt Series (Pixel Size 0.39 nm), 3.4 GB

aba2015-08-12-11_slicer14161.png, unhappy-inner-membrane, 563.6 kB

Files

aba2015-08-12-11_slicer14161.png

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Additional details

Identifiers

DOI
10.22002/yxf76-xj227
TILT_SERIES_ID
aba2015-08-12-11

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2015-08-12
Created
2015-08-12
Updated
2015-09-21