Published 2009 | Version v1
Dataset Open

IST1N tubes

Creators

  • 1. ROR icon California Institute of Technology

Contributors

Researcher:

Description

IST1N, tubes, ESCRT

Technical info

Tilt Series Date: 2009-11-11

Data Taken By: Megan Dobro

Species / Specimen: IST1N tubes

Strain:

Tilt Series Settings: Single Axis, tilt range: (60.0°, 60.0°), step: 2 degrees°, constant angular increment, dosage: 1.0 eV/Ų, defocus: 3.5 μm, magnification: 41000x.

Acquisition Software: Leginon

Upload Method: webload

Processing Software Used: Raptor

Collaborators and Roles: John McCullough purified in Utah

Purification / Growth Conditions / Treatment: Dialysis step after receiving protein

Sample Preparation: Vitrobot, varying blot times and offsets, 10 nm gold, 100 % humidity, 22 degrees

Attached Files

09nov11b_Box380grid2_003.mrc, Tilt Series (Pixel Size 0.51 nm), 520.1 MB

09nov11b_Box380grid2_003_full.rec, Reconstruction (Pixel Size 0.51 nm), 513.8 MB

Files

keyimg_mdj2009-11-11-2.jpg

Files (1.1 GB)

Name Size
520.1 MB (external) Download
513.8 MB (external) Download
md5:a1dcb3a691fc98c14e5979e1fe2c24a3
1.3 MB Preview Download
md5:aebf9af397aae4ba4bc1cc1095c2af35
16.6 MB Preview Download

Additional details

Identifiers

DOI
10.22002/yxxs1-6hf02
TILT_SERIES_ID
mdj2009-11-11-2

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2009-11-11
Created
2010-01-21
Updated
2010-01-21