BacillusSubtilis_293

Description

Technical info

Tilt Series Date: 2020-02-16

Data Taken By: Mohammed Kaplan

Species / Specimen: Bacillus subtilis

Strain: AB293, ΔponA amyE::fliP-2XHA sacA::ymdB Δmbl

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 3°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -6.0 μm, magnification: 19500x.

Microscope: Caltech Titan Krios

Acquisition Software: Serial EM

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: The samples were prepared with Amit and Ritesh (from Sigal Ben Yehuda and Ilan Rosenshine . labs at the Hebrew University). These Samples were incubated for four hours on grids on plates

Purification / Growth Conditions / Treatment: Cells were streaked on an agar plate from a -80C stock and incubated at 37C. Next day, the . cells were inoculated in 3 ml of LB and grown at 37C to OD 0.8-1. Cells were then added to . grids on agar plates and incubated for 4 hours at 37 C. The grids were washed with PBS and . then blotted and plunge frozen.

Sample Preparation: The sample was manually blotted to increase the chance of finding an intercellular nanotube.

Attached Files

Bsubtilis_293_070_af.mrc, Tilt Series (Pixel Size 0.449 nm), 1.9 GB

Bsubtilis_293_070_af_full.rec, Reconstruction (Pixel Size 1.796 nm), 737.3 MB