Published 2014 | Version v1
Dataset Open

Vibrio deltaCheA2

  • 1. ROR icon California Institute of Technology

Contributors

Researcher:

Description

V. cholerae strain deleted for CheA2

Technical info

Tilt Series Date: 2014-04-10

Data Taken By: Ariane Briegel

Species / Specimen: Vibrio cholerae

Strain: O1 biovar El Tor

Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 160.0 eV/Ų, defocus: -10.0 μm, magnification: 34000x.

Microscope: Caltech Polara

Acquisition Software: UCSFTomo

Upload Method: pipeline

Processing Software Used: Raptor

Collaborators and Roles: Ikuro Kawagishi

Purification / Growth Conditions / Treatment: Grew in LB 24 hours at 30C; diluted 150 µL into 2 mL Ca-HEPES buffer and grew 16 hours at 30C

aba2014-04-10-8_slicer3820.jpg: flagellar sheath comming off, motor in delta CheA2 strain aba2014-04-10-8_slicer3821.jpg: short polar array delta CheA2 strain aba2014-04-10-8_slicer3822.jpg: fillament sheet delta CheA2 strain

Attached Files

VibrioDeltaA20006.mrc, Tilt Series (Pixel Size 0.32 nm), 3.4 GB

VibrioDeltaA20006_full.rec, Reconstruction (Pixel Size 1.28 nm), 712.7 MB

Files

aba2014-04-10-8_slicer3820.jpg

Files (4.2 GB)

Name Size
md5:d591cefbc97aef8bddde5a50465dffc7
194.0 kB Preview Download
md5:a3c9b74b6885c2fec3c9baa0d4aafad1
205.4 kB Preview Download
md5:bcf1abd957f09a7c63bf8a16cba4004e
147.2 kB Preview Download
md5:a4dfc9f96c1bb30bafa3d6657032f6a6
1.1 MB Preview Download
md5:76f5631123fca9010889bf758185f67d
27.7 MB Preview Download
3.4 GB (external) Download
712.7 MB (external) Download

Additional details

Identifiers

DOI
10.22002/yzdy6-45d12
TILT_SERIES_ID
aba2014-04-10-8

Funding

NIH
HHMI
Beckman Institute
Gordon and Betty Moore Foundation
Agouron Institute
John Templeton Foundation

Dates

Collected
2014-04-10
Created
2014-04-10
Updated
2014-09-24